Mills Laboratory > Daniel Garrido

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Daniel Garrido

Daniel Garrido picFORMER POSTDOCTORAL RESEARCHER

 

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RESEARCH INTERESTS

My research at the Mills lab is related to the functional characterization of genetic determinants in the consumption of human milk oligosaccharides, and other milk glycoconjugates, by the infant borne bifidobacteria. This is an interesting model of evolution that can be used for diverse applications in the food industry.

More broadly, my research interests are studies at the molecular level and applications of probiotics and prebiotics in the food industry, including genomic and transcriptomic tools; and studies of mammalian milks and the intestinal microbiota as a source of bioactive agents with a potential impact on human health.

 

EDUCATION

• Ph.D in Food Science, Designated Emphasis in Biotechnology. University of California, Davis 2012.

• B.Sc. Engineering in Molecular Biotechnology. University of Chile, Santiago. 2005

 

HONORS AND AWARDS

  • National Milk Producers Federation (NMPF) National Dairy Leadership Scholarship Award. July 2011.
  • Fulbright-CONICYT doctoral scholarship in the U.S. Ministry of Education and Fulbright Chile Commission, 2006-2011.
  • Student Travel Award, First International Conference on the Glycobiology of Human Milk Oligosaccharides. Copenhagen, Denmark May 16-17 2011.
  • RMI Graduate Student Poster Competition. 3rd place. January 30th 2011. Robert Mondavi Institute, UC Davis.
  • Student Travel Award, International Milk Genomics Consortium, Davis CA October 20th 2010.
  • Graduate Student Travel Award, UC Davis Graduate Council. May 21st 2010.
  • Jastro-Shields Research Scholarship. Dept. of Food Science, UC Davis 2009
  • Highest Ranked Student in the Class of 2003, Engineering in Molecular Biotechnology

 

PUBLICATIONS

  • Garrido, D. A., D. C. Dallas and D. A. Mills.  2013. Consumption of human milk glycoconjugates by infant-associated bifidobacteria: Mechanisms and implications.  Microbiology (In Press).
  • Kim, J.-H., H. J. An, D. A. Garrido, J. B. German, C. B. Lebrilla,  D. A. Mills.  2013. Proteomic analysis of Bifidobacterium longum subsp. infantis reveals the metabolic insight on consumption of prebiotics and host glycans.  PLoS One (In Press).
  • Garrido, D., S. Ruiz-Moyano, J. Jimenez-Espinoza, H-J. Eom, D. E. Block and D. A. Mills.  2013. Utilization of galactooligosaccharides by Bifidobacterium longum subsp. infantis isolates. Food Microbiology 33:262–270.
  • Garrido, D., C. Nwosu, S. Ruiz-Moyano, D. Aldredge, J. B. German, C. B. Lebrilla and D. A. Mills. 2012.   Endo-beta-N-acetylglucosaminidases from infant-gut associated bifidobacteria release complex N-glycans from human milk glycoproteins. Molecular and Cellular Proteomics 11:775-785. Article press coverage
  • Garrido, D., S. Ruiz-Moyano and D. A. Mills. 2012.   Release and utilization of N-acetyl-D-glucosamine from human milk oligosaccharides by Bifidobacterium longumsubsp. infantis.  Anaerobe 18:430-435.
  • Garrido, D., D. Barile and D. A. Mills.  2012. A molecular basis for bifidobacterial enrichment in the infant gastrointestinal tract.  Advances in Nutrition 3:415S-421S.
  • Sela*, D. A., D. Garrido*, L. Lerno, S. Wu, K. Tan, H-J. Eom, A. Joachimiak, C. B. Lebrilla and D. A. Mills. 2012.  Bifidobacterium longum subsp. infantis ATCC15697 α-fucosidases are active on fucosylated human milk oligosaccharides.  Applied and Environmental Microbiology 78:795-803. (*authors contributed equally to this work)
  • Garrido, D., J.-H. Kim, J. B. German, H. E. Raybould, and D. A. Mills. 2011. Oligosaccharide binding proteins from Bifidobacterium longum subsp.infantis reveal a preference for host glycans.  PLoS ONE 6:e175315.
  • Godoy, L., Garrido, D., Martínez, C., Saavedra, J., Combina, M., Ganga, M.A. Study of the coumarate decarboxylase and vinylphenol reductase activities of Dekkera bruxellensis (anamorph Brettanomyces bruxellensis) isolates. Lett. Appl. Microbiol. 2009. 48: 452-457.
  • Salinas, F., Garrido, D., Ganga, A., Veliz, G., Martínez, C. Taqman real-time PCR for the detection and enumeration of Saccharomyces cerevisiae in wine. Food Microbiol. 2009. 26: 328-332.
  • Garrido D, Suau A, Pochart P, Cruchet S, Gotteland M (2005) Modulation of the fecal microbiota by the intake of a Lactobacillus johnsonii La1-containing product in human volunteers. FEMS Microbiology Letters 248: 249-256.
  • Brunser O, Gotteland M, Cruchet S, Figueroa G, Garrido D, et al. (2006) Effect of a milk formula with prebiotics on the intestinal microbiota of infants after an antibiotic treatment. Pediatric Research 59: 451-456.
  • Mangin I, Suau A, Magne F, Garrido D, Gotteland M, et al. (2006). Characterization of human intestinal bifidobacteria using competitive PCR and PCR-TTGE. FEMS Microbiology Ecology 55: 28-37.

 

PRESENTATIONS (Oral and Poster)

  • Garrido, D.  New insights into the molecular adaptations of infant-associated bifidobacteria to human milk glycoconjugates. Oral presentation, 8th UC Davis Retreat in Host-Microbe Interactions, Lake Tahoe, CA, October 2012.
  • “Glycosyl hydrolases implicated in the consumption of human milk oligosaccharides in infant gut associated bifidobacteria”. Young investigator oral presentation. 111th Annual Meeting of the American Society of Microbiology, San Francisco, CA, June 2012.
  • “Bifidobacterial Endoglycosidases Release Complex N-linked Glycans from Host Glycoproteins”. Experimental Biology 2012, San Diego CA April 2012.
  • “Breast milk sugars and the selection of beneficial bacteria in the infant gut”. Encuentros 2011, Berkeley, CA, November 2011.
  • “Characterization of Glycosyl Hydrolases in Bifidobacterium infantis active on Human Milk Oligosaccharides”. Oral presentation. First International Conference on the Glycobiology of Human Milk Oligosaccharides. Copenhagen, Denmark May 16-17 2011.
  • “A Preference for Host Derived Glycans in Bifidobacterium longum subsp. infantis”. 3rd ASM meeting in Beneficial Microbes. Miami, FL, October 25-29 2010.
  • “Human milk oligosaccharides import in Bifidobacterium longum subsp. infantis”. 2nd UC Davis Retreat in Host-Microbe Interactions, Lake Tahoe, CA, September 2010.
  • “Functional Characterization of Extracellular Solute Binding Proteins from Probiotic Bifidobacteria”. Annual Meeting of the Institute of Food Technology, Anaheim, CA May 2009.
  • “Functional characterization of extracellular glycan binding proteins from Bifidobacterium longum subsp. infantis: Role in Transport of Human Milk Oligosaccharides and Adhesion to Host Cells”. 109th Annual Meeting of the American Society of Microbiology, in Philadelphia, PA, May 2009.
  • “Development of a quantitive PCR protocol for the quantification of Brettanomyces bruxellensis yeasts in wine” XXVIII Chilean Congress of Microbiology. November 2006, Pucon, Chile
  • “Genetic analysis of strains of Brettanomyces bruxellensis of different geographical origin”. XXVII Chilean Congress of Microbiology. October 2005, Pucón, Chile.
  • “Evaluation by FISH and flow cytometry of the effect on antibiotic administration on the intestinal microflora of children”. Oral presentation, LXV Congress of the Gastroenterology Society of Chile. December 2003, Viña del Mar, Chile.
  • “Evaluation by FISH and flow cytometry of the changes induced in the fecal microbiota by the intake of Lactobacillus johnsonii La1”. II Argentinian-Italian Symposium on lactic acid bacteria. November 2004, San Miguel de Tucumán, Argentina.
  • “Quantitation of Lactobacillus sp. and L. johnsonii by competitive PCR” II Argentinian-Italian Symposium on lactic acid bacteria. November 2004, San Miguel de Tucumán, Argentina.
  • “Effect of an infant formula with prebiotics on the intestinal microbiota after an antibiotic treatment”. LXVI Congress of the Gastroenterology Society of Chile. December 2004, Viña del Mar, Chile.
  • Evaluation by FISH and flow cytometry of the changes induced on the fecal microbiota by the intake of Lactobacillus johnsonii La1”. LXVI Congress of the Gastroenterology Society of Chile. December 2004, Viña del Mar, Chile

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